Akeagen CaMouse
Transforming the way
single domain antibodies are discovered
About Akeagen, Inc.
Our Mission
Our vision is to transform the way antibodies are discovered — empowering researchers, innovators, and partners to shorten the distance between an idea and a therapy. By integrating cutting-edge platforms, from humanized antibody mice to single-domain antibody discovery, we aim to make the pursuit of cures faster, smarter, and more accessible.
Revolutionizing Single Domain Antibody Discovery
Akeagen CaMouse Platform:
CaMouse is Akeagen's proprietary genetically engineered mouse platform designed to generate single domain antibodies (sdAbs) directly from immunization:
- The mouse heavy chain locus is engineered that mimic camelid heavy chain locus.
- preserve native V(D)J recombination, somatic hypermutation, and in vivo selection and natural affinity maturation.
- 144 VH genes from mouse to maximized VHH repertoire and diversity.
No camels and no farm are needed, CaMouse (Camel Mouse) will help your single domain antibody discovery process more efficient, faster, accessible all in your lab settings!
Single Domain Antibodies
Single domain antibodies (sdAbs, also known as VHH, Nanobodies), the variable domain of Heavy-Chain-Only antibodies (HcAbs), are the smallest functional antigen-binding fragment of an antibody in nature. HcAbs are special type of antibodies naturally found in camelids (llama, apaca, camel) and some cartilaginous fish (sharks, nurse sharks, etc.). Unlike conventional IgG, they completely lake light chain.
Advantages of Single Domain Antibodies
1. Small Binding Domains --- VHH (15 kDa) are 1/10 the size of IgG.
2. High Stability --- Soluble, resistant to heat, pH, and proteases.
3. Unique Epitope Access --- Long CDR loops can penetrate clefts, enzyme pockets, or GPCR
active sites.
4. Easy Engineering --- Modular and simple to fuse into bispecifics, CAR-T constructs, or half-
extended formats.
5. Simple Expression --- Can be produced in bacteria, yeast, or mammalian systems.
Applications
1. Therapeutics
- Autoimmune & inflammation: anti-TNF, anti-IL-17 nanobody drugs.
- Oncology: bispecific nanobody T cell engagers, CAR-T binders, nanobody-drug-conjugates.
- Infectious diseases: influenza and SARS-CoV-2 neutralizing nanobodies
2. Theragnostics and Imagining
- personalized medicine that uses s single molecule to both diagnose and treat a disease:
- Radiopharmaceutical targeting PD-L1, DLL3 etc.
3. Research Reagents
- intrabody
- single molecule live-cell imaging
- 3D tissue imaging
4: Drug Delivery
- tLNP targeted lipid nanoparticles conjugated with nanobody for tissue targeted delivery in vivo
- nanobody conjugated lentiviral vector for in vivo CAR-T